Introduction of ID2 Enhances Invasiveness in ID2-null Oral Squamous Cell Carcinoma Cells via the SNAIL Axis

Cancer Genomics - Proteomics

TOMOKI SUMIDA²  YOSHIHIDE MORI²  YU KAMATA²  WATARU KUMAMARU²  AKIKO ISHIKAWA¹  YOSUKE KOBAYASHI² 

[1]epartment of Oral and Maxillofacial Surgery, Ehime University Graduate School of Medicine, Ehime, Japanepartment of Oral and Maxillofacial Surgery, Ehime University Graduate School of Medicine, Ehime, Japanepartment of Oral and Maxillofacial Surgery, Ehime University Graduate School of Medicine, Ehime, Japan

[2]ection of Oral & Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japanection of Oral & Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japanection of Oral & Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

关键词: Inhibitor of differentiation;    epithelial–mesenchymal transition;    matrix metalloproteinase;    invasion;    SNAIL;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Aim: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and Methods: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results: ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial–mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion: ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2–SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC.

【 授权许可】

Unknown