Dual Roles of 17-β Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma

Cancer Genomics - Proteomics

HAN-SUN CHIANG⁶  CHIH-MING LIN⁷  SHAO-KUAN CHEN³  KUO-CHIANG CHEN¹  WEI-CHI KU⁵  SHENG-TANG WU²  CHI-JUNG HUANG⁴ 

[1] raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.raduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.;ivision of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.ivision of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.ivision of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.;epartment of Surgery, Sijhih Cathay General Hospital, New Taipei, Taiwan, R.O.C.epartment of Surgery, Sijhih Cathay General Hospital, New Taipei, Taiwan, R.O.C.epartment of Surgery, Sijhih Cathay General Hospital, New Taipei, Taiwan, R.O.C.;chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.epartment of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.epartment of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.;chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.chool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.;raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Urology, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Urology, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.epartment of Urology, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.raduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C.epartment of Urology, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.;epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.epartment of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C.

关键词: 17-β-estradiol;    autophagy;    estrogen receptor;    apoptosis;    quantitative phosphoproteomics;    renal cell carcinoma;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: It has been proposed that 17-β-estradiol (E2) activates estrogen receptor and inhibits renal cell carcinoma (RCC) growth. In the present study we explored the role of E2 and ER in the regulation of RCC growth. Materials and Methods: The RCC cell line ACHN was treated by E2 combining with E2 antagonist Fulvestrant or ER knockdown, and cell growth was monitored. Quantitative phosphoproteomics was applied to study the E2 regulated non-genomic phosphorylation changes. Western blotting, immunofluorescence microscopy, and apoptosis assays were used for validation. Results: E2 induced ER-dependent growth inhibition in RCC cell lines. Quantitative phosphoproteomics revealed that E2 induced both apoptosis and autophagy. Cellular apoptosis was confirmed by altered mitochondrial membrane potential, and ER-dependent autophagosome formation was also found. Conclusion: Our data revealed the potential dual roles of E2 in regulating RCC growth via autophagy and apoptosis pathways.

【 授权许可】

Unknown