Cancer Genomics - Proteomics | |
WNT/β-catenin Signaling Pathway and Downstream Modulators in Low- and High-grade Glioma | |
TETYANA DENYSENKO4  ANTONIO MELCARNE2  MARTA MELLAI3  PAOLA CASSONI1  LAURA ANNOVAZZI3  DAVIDE SCHIFFER3  | |
[1] epartment of Medical Sciences, University of Turin/Health and Science City, Turin, Italyepartment of Medical Sciences, University of Turin/Health and Science City, Turin, Italyepartment of Medical Sciences, University of Turin/Health and Science City, Turin, Italy;epartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italy;esearch Center, Polyclinic of Monza Foundation, Vercelli, Italyesearch Center, Polyclinic of Monza Foundation, Vercelli, Italyesearch Center, Polyclinic of Monza Foundation, Vercelli, Italy;esearch Center, Polyclinic of Monza Foundation, Vercelli, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italyesearch Center, Polyclinic of Monza Foundation, Vercelli, Italyesearch Center, Polyclinic of Monza Foundation, Vercelli, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italyesearch Center, Polyclinic of Monza Foundation, Vercelli, Italyepartment of Neurosurgery, CTO Hospital/Health and Science City, Turin, Italy | |
关键词: Gliomas; glioblastoma multiforme; WNT3a; β-catenin; TCF4; prognosis; | |
DOI : | |
来源: Delinasios GJ CO | |
【 摘 要 】
Background: Aberrant activation of the canonical Wingless-type MMTV integration site family (WNT)/β-catenin signaling pathway is critical for gliomas. Materials and Methods: In 74 gliomas of different histological grade and in 24 glioblastoma cell lines, protein expression of WNT member 3a (WNT3a), β-catenin and transcription factor 4 (TCF4) was investigated by immunohistochemistry, western blotting, immunofluorescence and immunocytochemistry. In tumors and cell lines, WNT3A expression was assessed at the mRNA level by quantitative real-time polymerase chain reaction. Results: WNT3a was overexpressed at the protein and mRNA levels in malignant astrocytic tumors and cell lines. Cytoplasmic expression of β-catenin was detected in high-grade gliomas and cell lines, with evidence of nuclear translocation on fractionated protein extracts. Activating mutations in the β-catenin encoding gene (CTNNB1) were excluded by direct sequencing. TCF4 was statistically correlated with Ki-67/MIB-1 and cyclin D1 labeling indices. Conclusion: Expression of WNT3a, cytoplasmic β-catenin and TCF4 was significantly associated with the histological malignancy grade and with a worse prognosis for patients with glioma.
【 授权许可】
Unknown
【 预 览 】
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RO201912010183855ZK.pdf | 1026KB | download |