Isolation of Stem Cells Using Spheroids from Fresh Surgical Specimen: An Analytic Mini-Review

Cancer Genomics - Proteomics

YAN GAO MAN¹  HONGJIAN WANG²  ITZHAK AVITAL¹  JINLIAN WANG⁴  WILLIAMS CS CHO³  ALEXANDER STOJADINOVIC¹  CIARAN MANNION⁵ 

[1] Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.;College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou, P.R. ChinaCollege of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou, P.R. ChinaCollege of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou, P.R. China;Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, P.R. ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, P.R. ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, P.R. China;Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, U.S.A.Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, U.S.A.Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, U.S.A.;Department of Pathology, Hackensack University Medical Center, Hackensack, NJ, U.S.A.Department of Pathology, Hackensack University Medical Center, Hackensack, NJ, U.S.A.Department of Pathology, Hackensack University Medical Center, Hackensack, NJ, U.S.A.

关键词: Adult stem cells;    cellular replenishment;    carcinogenesis;    spheroid;    surgical specimens;    review;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

It is a commonly held belief that adult stem cells represent the “seeds” for normal cellular replenishment and also for carcinogenesis. The identification and characterization of stem cells for clinical therapeutic applications, however, is extremely challenging for a number of reasons. Recently, our group and others have attempted to isolate stem cells using spheroids from fresh surgical specimens and utilize them for in vitro and in vivo studies. This mini-review summarizes the major technical steps of these methods along with the primary findings. Besides, it critically analyzes the advantages and limitations of the concept and technical approaches. Finally, this mini-review presents our thoughts on the potential future directions of stem cell isolation and cancer stem cell-related research and clinical applications.

【 授权许可】

Unknown