Guanine Nucleotide Binding Protein β 1: A Novel Transduction Protein with a Possible Role in Human Breast Cancer

Cancer Genomics - Proteomics

ANUP K. SHARMA¹  WEN G. JIANG²  UMAR WAZIR¹  KEFAH MOKBEL³ 

[1] Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.;Metastasis and Angiogenesis Research Group, University Department of Surgery, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff, Wales, U.KMetastasis and Angiogenesis Research Group, University Department of Surgery, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff, Wales, U.KMetastasis and Angiogenesis Research Group, University Department of Surgery, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff, Wales, U.K;Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.The London Breast Institute, Princess Grace Hospital, London, U.KDepartment of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.The London Breast Institute, Princess Grace Hospital, London, U.KThe London Breast Institute, Princess Grace Hospital, London, U.KDepartment of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.The London Breast Institute, Princess Grace Hospital, London, U.K

关键词: Breast cancer;    apoptosis;    GNB1;    tumour suppressor gene;    PCR;    autophagy;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

To our knowledge, this is the first study to examine the relationship between guanine nucleotide binding protein β-1 (GNB1) mRNA expression and clinicopathological parameters. Furthermore, the correlations between GNB1, Rictor and the mammalian target of rapamycin (mTOR) were also investigated. Materials and Methods: Breast cancer tissues (n=136) and normal tissues (n=31) underwent reverse transcription and quantitative polymerase chain reaction. Transcript levels were correlated with clinicopathological data. Results: Higher mRNA transcript levels of GNB1 were found in the breast cancer specimens in paired samples (p=0.0029). The mRNA expression of GNB1 increased with TNM stage (TNM1 vs. TNM2/3/4, p=0.036), tumour grade (grade 2 vs. 3, p=0.006), in ductal tumours (p=0.0081), and was associated with adverse patient outcomes (mortality vs. disease-free survival: 4.9 vs. 0.01, p=0.027). GNB1 was positively-correlated with mTOR (r=0.525, p<0.000001) and Rictor (r=0.388, p=0.0000606). Conclusion: These observations may suggest that GNB1 plays an important role in the mTOR-related anti-apoptosis pathway and can potentially be targeted in the treatment of human breast cancer.

【 授权许可】

Unknown