Cancer Genomics - Proteomics | |
MicroRNA Expression Profile of MCF-7 Human Breast Cancer Cells and the Effect of Green Tea Polyphenon-60 | |
MARY A. FARWELL2  MAITRI SHAH2  LINDSEY N. FIX2  THOMAS EFFERTH1  BAOHONG ZHANG2  | |
[1] Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 55128 Mainz, GermanyDepartment of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 55128 Mainz, GermanyDepartment of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 55128 Mainz, Germany;Department of Biology, East Carolina University, Greenville, NC 27858, U.S.A.Department of Biology, East Carolina University, Greenville, NC 27858, U.S.A.Department of Biology, East Carolina University, Greenville, NC 27858, U.S.A. | |
关键词: Green tea; micro-RNA; microarray; gene expression; Polyphenon-60; breast cancer; MCF-7; | |
DOI : | |
来源: Delinasios GJ CO | |
【 摘 要 】
This study reports for the first time the microRNA expression profile of human breast cancer MCF-7 cells and the effect of green tea. Although hundreds of miRNAs have been identified in humans, only a small proportion (25.6%) of miRNAs are expressed in MCF-7 cells. Low concentration treatment with Polyphenon-60 significantly alters the miRNA expression profile in MCF-7 cells. Twenty three miRNAs have been identified with differential expression after a 48 h treatment with 10 μg/ml Polyphenon-60 (green tea extract). These miRNAs include miR-21 and miR-27 that were found to be down-regulated following treatment with green tea. These two miRNAs have previously been identified as being overexpressed in MCF-7 breast cancer cells, with miR-21 specifically implicated in down-regulating the tumor suppressor gene, tropomyosin-1. This data supports the hypothesis that Polyphenon-60-induced modification of the breast cancer miRNA expression profile contributes to the efficacy of green tea treatment. The resulting decrease in carcinogenesis is further supported by the altered miRNA regulation of potential oncogenes and tumor-suppressor genes.
【 授权许可】
Unknown
【 预 览 】
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RO201912010183690ZK.pdf | 414KB | download |