Gene Expression Profile by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin in the Liver of Wild-Type (AhR+/+) and Aryl Hydrocarbon Receptor-Deficient (AhR-/-) Mice

Journal of Veterinary Medical Science

Bang Hyun KIM³  Mun Suk PARK³  Chang Yong YOON³  Yong Soon LEE¹  Ji Yeon PARK³  Beom Jun LEE²  HoIl KANG³  Hyugsung KWON³  Misun PARK³  Youn Kyoung JEONG³  Hai Kwan JUNG³ 

[1] Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University;Department of Veterinary Public Health, Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungbuk National University;Department of Toxicology, National Institute of Toxicological Research

关键词: gene expression;    liver;    microarray;    TCDD;   

DOI  :  10.1292/jvms.68.663

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(43)Cited-By(6)2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is one of the most toxic environmental pollutants that cause various biological effects on mammals. The purpose of our study was to identify the genes involved in hepatotoxicity and hepatocarcinogenesis caused by TCDD. C57BL/6 (AhR+/+, wild type) and B6.129-AhR/J (AhR-/-, knock out) mice were injected i.p. with a single treatment of TCDD at the dose of 100 μg/kg body weight. Relative liver weight was significantly increased at 72 hr after TCDD treatment without an apparent histopathological change in AhR+/+ mice (p<0.05). TCDD treatment also significantly increased activity of serum alanine aminotransferase in AhR-/- mice (p<0.05). The liver was analyzed for gene expression profiles 72 hr later. As compared with AhR-/- mice, the expression of 51 genes (>3-fold) was changed in AhR+/+ mice; 28 genes were induced, while 23 genes were repressed. Most of the genes were associated with chemotaxis, inflammation, carcinogenesis, acute-phase response, immune responses, cell metabolism, cell proliferation, signal transduction, and tumor suppression. This study suggests that the microarray analysis of genes in the liver of AhR+/+ and AhR-/- mice may help to clarify the mechanism of AhR-mediated hepatotoxicity and hepatocarcinogenesis by TCDD.

【 授权许可】

Unknown