Galectin-3 Expression in Human Papillary Thyroid Carcinoma

Cancer Genomics - Proteomics

JISNUSON SVASTI²  CHANTRAGAN SRISOMSAP¹  PHANNEE SAWANGAREETRAKUL³  DARANEE CHOKCHAICHAMNANKIT³ 

[1] aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210;aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210epartment of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210epartment of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailandhulabhorn Graduate Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210epartment of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailandepartment of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210hulabhorn Graduate Institute, Bangkok 10210epartment of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailand;aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210

关键词: Galectin-3;    thyroid diseases;    proteomics;    immunodetection;    papillary thyroid carcinoma;    LC/MS/MS;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Previous studies have suggested that galectin-3 expression was markedly elevated in papillary thyroid carcinoma compared to other thyroid diseases. In order to better understand this protein, galectin-3 from papillary thyroid carcinoma was partially purified by affinity chromatography on lactose-agarose. Proteins eluted from the column were separated by SDS-PAGE, and galectin-3 was detected with antibodies against the N-terminus and C-terminus of galectin-3. Some protein bands from the lactose binding fraction were also selected for identification by liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). Seven protein bands, with molecular weights ranging from 16 kDa to 31 kDa, were identified as galectin-3. The antibody raised against the C-terminus of galectin-3 gave a strong band for one of the bands detected by the N-terminal antibody and weak bands for the other three. One additional dark immunoreactive band with an approximate molecular weight of 20 kDa, was also detected by the C-terminal galectin-3 antibody. To determine the structural differences of each protein band, N-terminal amino acid sequencing of the seven protein bands was conducted. The three upper bands were N-terminally blocked, while the other bands had N-terminal amino acid sequences starting at positions Gly35, Gly65 (2 bands) and Ala100, respectively. Further studies are necessary to determine whether these are due to nonspecific proteolysis or post-translation modification

【 授权许可】

Unknown