Characterization of Microarray Gene Expression Profiles of Early Stage Thyroid Tumours

Cancer Genomics - Proteomics

TÍMEA VARJAS¹  ISTVÁN KISS¹  ISTVÁN SZANYI⁴  KATALIN GOMBOS¹  ESZTER SZELE¹  ISTVÁN EMBER¹  LÁSZLÓ PUSKÁS⁵  ERIK KOVÁCS⁶  FERENC JUHÁSZ²  LÁSZLÓ KOZMA³ 

[1] nstitute of Public Health, Faculty of Medicine, University of Pécs, Pécsnstitute of Public Health, Faculty of Medicine, University of Pécs, Pécsnstitute of Public Health, Faculty of Medicine, University of Pécs, Pécs;irst Department of Surgery, University of Debrecen, Faculty of Medicine, Debrecen, Hungaryirst Department of Surgery, University of Debrecen, Faculty of Medicine, Debrecen, Hungaryirst Department of Surgery, University of Debrecen, Faculty of Medicine, Debrecen, Hungary;egional Blood Transfusion Center of Debrecen, Debrecenegional Blood Transfusion Center of Debrecen, Debrecenegional Blood Transfusion Center of Debrecen, Debrecen;epartment of Otolaryngology Head and Neck Surgery, Faculty of Medicine, University of Pécs, Pécsepartment of Otolaryngology Head and Neck Surgery, Faculty of Medicine, University of Pécs, Pécsepartment of Otolaryngology Head and Neck Surgery, Faculty of Medicine, University of Pécs, Pécs;unctional Genomical Laboratory, Biological Research Center of Hungarian Academy of Sciences, Szegedunctional Genomical Laboratory, Biological Research Center of Hungarian Academy of Sciences, Szegedunctional Genomical Laboratory, Biological Research Center of Hungarian Academy of Sciences, Szeged;eart Institute and First Department of Internal Medicine, University of Pécs, Pécseart Institute and First Department of Internal Medicine, University of Pécs, Pécseart Institute and First Department of Internal Medicine, University of Pécs, Pécs

关键词: Microarray;    gene expression profiling;    thyroid tumour;    follicular adenoma;    follicular carcinoma;    papillary carcinoma;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Microarray analysis offers the opportunity of screening transcriptional expression profile of neoplastic cells on a genomic level. Defining consistent changes in gene expression pattern of tumours enables the detection of genes essential for tumorigenesis and might provide biomarkers to early recognition of malignant behaviour and new therapeutical targets. Patients and Methods: A high-density oligonucleotide array with 20,000 human gene-specific oligonucleotide was used to analyze benign and early-stage malignant thyroid tumours of epithelial origin: follicular adenoma, follicular carcinoma and papillary carcinoma, compared to normal thyroid tissue. Results: Significant expression differences of 279 genes - underexpression of 252 and overexpression of 27 genes - were found. The overlapping genes of the different histological types were examined extensively. Among these genes a limited set acting on the same transcriptional pathway, through NF-ÃŽB, were found. Conclusion: The role of overlapping genes in histologically different tumours has not been clarified, but might represent early or pivotal steps of carcinogenesis. All investigated histiotypes of tumours contained significantly modulated genes acting on the NF-ÃŽB regulatory pathway. Our findings suggest that modulation of NF-ÃŽB signalling plays a crucial role in early thyroid carcinogenesis.

【 授权许可】

Unknown