Concurrent Down-regulation of Egr-1 and Gelsolin in the Majority of Human Breast Cancer Cells

Cancer Genomics - Proteomics

JINGBO LIU²  SHIYONG LI⁴  RUOCHUN HUANG²  WEIMIN YANG²  RUO-PAN HUANG²  DONGZI YANG¹  CHEN YAO³  YA-GUANG LIU² 

[1] epartment of Gynecology and Obstetrics, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. Chinaepartment of Gynecology and Obstetrics, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. Chinaepartment of Gynecology and Obstetrics, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. China;epartment of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, U.S.A.epartment of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, U.S.A.epartment of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, U.S.A.;epartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, U.S.A.epartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, U.S.A.epartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, U.S.A.;epartment of Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. Chinaepartment of Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. Chinaepartment of Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. China

关键词: Egr-1;    gelsolin;    breast cancer;    gene expression;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

A growing body of evidence suggests that early growth response-1 (Egr-1), a transcription factor, may function as a tumor suppressor. The aim of this study was to gain more evidence to support the role of Egr-1 in the suppression of cancer cell growth and to examine the potential correlation between Egr-1 and gelsolin. Materials and Methods: Histochemical staining coupled with breast cancer tissue arrays were used to examine the expression levels of Egr-1 and gelsolin. Reporter assays and gel shift were used to study the transcriptional activity of Egr-1 on the regulation of gelsolin. Results: Our data showed that most normal mammary tissues expressed high levels of Egr-1, while the majority of breast cancer tissues expressed very small amounts of Egr-1. The expression pattern of Egr-1 in human breast cancer tissues was highly correlated with gelsolin expression. Induction of Egr-1 by serum stimulation accompanied the increase of gelsolin expression. In cells lacking the induction of Egr-1 in response to serum stimulation, gelsolin expression remained unchanged. Furthermore, gelsolin promoter activity was profoundly reduced in Egr-1 null mouse embryonic fibroblasts compared to Egr-1 wild-type mouse embryonic fibroblasts. Gel shift experiments indicated that Egr-1 can directly bind to the gelsolin promoter. Conclusion: Our results suggest that Egr-1 may be an important breast cancer marker and that an as yet uncharacterized pathway involved in Egr-1 and gelsolin expression exists which leads to breast cancer cell development.

【 授权许可】

Unknown