International Journal of Molecular Sciences | |
Egr-1 Upregulates Siva-1 Expression and Induces Cardiac Fibroblast Apoptosis | |
Karin Zins2  Jiri Pomyje1  Erhard Hofer1  Dietmar Abraham2  Trevor Lucas2  | |
[1] Molecular Vascular Biology, Department of Vascular Biology and Thrombosis Research, Vienna Competence Center, Vienna Medical University, Vienna A-1090, Austria; E-Mails:;Laboratory for Molecular Cellular Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna A-1090, Austria; E-Mails: | |
关键词: cardiac fibroblasts; apoptosis; gene expression; transcription factor; Egr-1; Siva-1; | |
DOI : 10.3390/ijms15011538 | |
来源: mdpi | |
【 摘 要 】
The early growth response transcription factor Egr-1 controls cell specific responses to proliferation, differentiation and apoptosis. Expression of Egr-1 and downstream transcription is closely controlled and cell specific upregulation induced by processes such as hypoxia and ischemia has been previously linked to multiple aspects of cardiovascular injury. In this study, we showed constitutive expression of Egr-1 in cultured human ventricular cardiac fibroblasts, used adenoviral mediated gene transfer to study the effects of continuous Egr-1 overexpression and studied downstream transcription by Western blotting, immunohistochemistry and siRNA transfection. Apoptosis was assessed by fluorescence microscopy and flow cytometry in the presence of caspase inhibitors. Overexpression of Egr-1 directly induced apoptosis associated with caspase activation in human cardiac fibroblast cultures
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland
【 预 览 】
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