Transcriptomic Molecular Markers for Screening Human Colon Cancer in Stool and Tissue

Cancer Genomics - Proteomics

STEFAN P. MARCUARD²  WADE NAZIRI⁵  DENNIS R. SINAR⁶  FARID E. AHMED³  DONALD T. LYSLE⁷  GORDON FLAKE⁸  RON R. ALLISON³  RODNEY PENNINGTON¹  STEPHANIE IJAMES⁷  PAUL VOS⁴ 

[1] oche Applied Science, Indianapolis, IN 46250, U.S.A.oche Applied Science, Indianapolis, IN 46250, U.S.A.oche Applied Science, Indianapolis, IN 46250, U.S.A.;arolina Digestive Disease, Greenville, NC 27858arolina Digestive Disease, Greenville, NC 27858arolina Digestive Disease, Greenville, NC 27858;epartment of Radiation Oncology, LSB 003, Leo W. Jenkins Cancer Center, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858epartment of Radiation Oncology, LSB 003, Leo W. Jenkins Cancer Center, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858epartment of Radiation Oncology, LSB 003, Leo W. Jenkins Cancer Center, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858;epartment of Biostatistics, School of Allied Health Sciences, ECU, Greenville, NC 27858epartment of Biostatistics, School of Allied Health Sciences, ECU, Greenville, NC 27858epartment of Biostatistics, School of Allied Health Sciences, ECU, Greenville, NC 27858;arolina Physicians PA, Greenville, NC 27834arolina Physicians PA, Greenville, NC 27834arolina Physicians PA, Greenville, NC 27834;epartment of Internal Medicine, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858epartment of Internal Medicine, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858epartment of Internal Medicine, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858;epartment of Psychology, University of North Carolina, Chapel Hill, NC 27599epartment of Psychology, University of North Carolina, Chapel Hill, NC 27599epartment of Psychology, University of North Carolina, Chapel Hill, NC 27599;aboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709aboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709aboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709

关键词: Adenocarcinoma;    colonocyte;    diagnosis;    IBD;    LCM;    genes;    QC;    RT-qPCR;    RNA;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

There is a need for sensitive and specific diagnostic molecular markers that can be used to monitor early patterns of gene expression in non-invasive exfoliated colonocytes shed in the stool, and in situ in adenoma-carcinoma epithelium of the colon. RNA-based detection methods are more comprehensive than either DNA-, protein- or methylation-based screening methods. By routinely and systematically being able to perform quantitative gene expression studies on these samples using less than ten colon cancer genes selected by the enormous resources of the National Cancer Institute's Cancer Genome Anatomy Project, we were able to monitor changes at various stages in the neoplastic process, allowing for reliable diagnostic screening of colon cancer particularly at the early, premalignant stages. Although the expression of some of the genes tested in tissue showed less variability in normal or cancerous patients than in stool, the stool by itself is suitable for screening. Thus, a transcriptomic approach using stool or tissue samples promises to offer more sensitivity and specificity than currently used molecular screening methods for colon cancer. A larger prospective clinical study utilizing stool and tissue samples derived from many control and colon cancer patients, to allow for a statistically valid analysis, is now urgently required to determine the true sensitivity and specificity of the transcriptomic screening approach for this preventable cancer.

【 授权许可】

Unknown