Application of Polymerase Chain Reaction to Analysis of Antigen Receptor Rearrangements to Support Endoscopic Diagnosis of Canine Alimentary Lymphoma

Journal of Veterinary Medical Science

Masaru OKUDA⁴  Takuya MIZUNO⁴  Tomoko TAKAHASHI¹  Toshihiro WATARI⁵  Yuko WADA⁴  Takako SHIMOKAWA MIYAMA⁴  Naoki KANEKO⁴  Kazuhito ITAMOTO²  Hiroko HIRAOKA⁴  Yoshifumi YAMAMOTO⁴  Munekazu NAKAICHI³ 

[1] Laboratory of Veterinary Radiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University;Laboratory of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Radiology, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University;Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

关键词: diagnosis;    enteric disease;    IBD;    Lymphoma/leukemia;    molecular identification;   

DOI  :  10.1292/jvms.71.555

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(12)We evaluated the usefulness of polymerase chain reaction for antigen receptor gene rearrangement analysis (PARR) of endoscopic biopsy specimens for diagnosis of canine alimentary lymphoma. Two endoscopic biopsy specimens were obtained from each lesion in 78 dogs with gastrointestinal symptoms. One specimen was histopathologically examined by a pathologist, and the other was analyzed by PARR. All samples were categorized into three groups [lymphoma (n=4), adenocarcinoma (n=5) and enteritis groups (n=69)] based on the histopathological diagnosis. In the lymphoma group, one case was IgH major-positive, and three cases were TCRγ-positive, representing clonal expansion of B- and T-cells, respectively. PARR produced negative results for all cases in the adenocarcinoma group. In the enteritis group, six cases were TCRγ-positive. Two of the six TCRγ-positive enteritis cases were cytologically diagnosed as lymphoma by fine needle aspiration during a laparotomy. In the enteritis group, the survival times were compared between the TCRγ-positive and TCRγ-negative cases. The overall survival time of the TCRγ-positive enteritis cases was significantly shorter than that of the TCRγ-negative enteritis cases according to a log-rank test (p<0.0001). With regard to other factors, such as age, clinical signs and the serum albumin concentration, there were no significant differences between the TCRγ-positive and TCRγ-negative enteritis cases. In conclusion, PARR is capable of detecting alimentary lymphoma and latent alimentary lymphoma, which cannot be histopathologically diagnosed using endoscopic biopsy specimens. Furthermore, a TCRγ-positive result in PARR may imply a poor prognosis.

【 授权许可】

Unknown