Molecular Assessment of the Tumor Protein Microenvironment Using Imaging Mass Spectrometry

Cancer Genomics - Proteomics

HERBERT S. SCHWARTZ⁴  ROBERT L. CALDWELL³  ADRIANA GONZALEZ²  RICHARD M. CAPRIOLI³  STACEY R. OPPENHEIMER¹ 

[1] ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.;epartment of Pathology, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Pathology, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Pathology, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.;ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.ass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.epartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.;anderbilt Orthopaedic Institute, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.anderbilt Orthopaedic Institute, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.anderbilt Orthopaedic Institute, Vanderbilt University School of Medicine, Nashville, TN, U.S.A.

关键词: Imaging mass spectrometry;    tumor microenvironment;    field effect;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Tumor cells or other bioreactive compounds derived from the tumor can migrate beyond the tumor margin and may be significant contributing factors in tumor recurrence. Local recurrence of cancer occurs quite often, despite microscopically-confirmed negative surgical resection margins, and is likely related to the presence of abnormal, fully neoplastic or pre-neoplastic cells adjacent to the tumor that cannot be recognized by conventional light microscopy. Identification of proteins that define the molecular tumor microenvironment of the neoplastic process may become an important part of the evaluation of cancer tissue, potentially helping guide surgical resection procedures. Tissue-based matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry is a molecular analysis technology that can significantly improve the assessment of tumor microenvironment at the molecular level. In the current study, this is illustrated with the analysis of an invasive soft tissue sarcoma. Marked differences in protein distribution between tumor and immediate adjacent tissue are shown, and these differences persist far into histologically normal appearing adjacent tissue. Several of these proteins are confirmed using immunohistochemistry. This study demonstrates that tissue-based MALDI imaging mass spectrometry is ideally suited for the molecular analysis of tumor microenvironment and could potentially augment histologic interpretation of tumor margins.

【 授权许可】

Unknown