Transient Expression of a Major Ampullate Spidroin 1 Gene Fragment from Euprosthenops sp. in Mammalian Cells

Cancer Genomics - Proteomics

HELENA NIMMERVOLL¹  ERICA STORCKENFELDT¹  STEFAN GRIP¹  NATALIA POUCHKINA-STANTCHEVA³  WILHELM ENGSTRÖM¹  SIMON J. MCQUEEN-MASON³  ANNA RISING¹  ARMANDO FERNANDEZ-ARIAS¹  FRITZ VOLLRATH² 

[1] epartment of Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Swedenepartment of Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Swedenepartment of Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Sweden;epartment of Zoology, University of Oxford, Sourth Parks Road, Oxford OX13PS, U.K.epartment of Zoology, University of Oxford, Sourth Parks Road, Oxford OX13PS, U.K.epartment of Zoology, University of Oxford, Sourth Parks Road, Oxford OX13PS, U.K.;NAP, Department of Biology, Area 8, University of York, York YO105YWNAP, Department of Biology, Area 8, University of York, York YO105YWNAP, Department of Biology, Area 8, University of York, York YO105YW

关键词: Spidroin 1 gene;    Euprosthenops;    mammalian cells;    spider silk protein;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Spider silk possesses extraordinary and unsurpassed mechanical properties and several attempts have been made to artificially produce spider silk in order to manufacture strong and light engineering composites. In the field of oncology, recombinant spider silk has the potential to be used as a biomaterial for bone replacement after tumour surgery. In this study, a 636-base pair gene fragment, coding for a part of major ampullate spidroin 1 from the African spider, Euprosthenops sp., was cloned into the expression vector pSecTag2/Hygro A, designed for the production of protein in mammalian cells. COS-1 cells were subsequently transfected with the recombinant plasmids and transient expression of low amounts of the corresponding silk protein fragment was obtained. The expressed fragment contained repetitive sequences associated with intrinsic biomechanical properties and has potential as a starting material for designed biopolymers.

【 授权许可】

Unknown