Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Processes

Stéphanie Tissot¹  Patrik O. Michel¹  Clara J. Douet¹  Sarah Grezet¹  Lucia Baldi¹  David L. Hacker¹ 

[1] Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, CH-1015 Lausanne, Switzerland;

关键词: shake flasks;    orbital shaking;    bacteria;    gas transfer;    insect cells;    mammalian cells;   

DOI  :  10.3390/pr1010003

来源: mdpi

【 摘 要 】

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (k_La) was 200%–400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube than in a 1-L Erlenmeyer flask. Mammalian cell cultures achieved a 25% higher cell density in the HT tube as compared to a 250-mL square-shaped bottle while insect cells grew as well in HT tubes as in 250-mL shake flasks. Because of their performance, disposability, and compact size, we conclude that 250-mL HT tubes are a useful alternative to other shaken containers for the cultivation of bacterial, mammalian and insect cells.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.