Defining the phenotype of neutrophils following reverse migration in zebrafish

Journal of Leukocyte Biology

Stephen A. Renshaw¹  Felix Ellett¹  Nikolay V. Ogryzko¹  Philip M. Elks¹  Anne L. Robertson¹ 

[1] Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United KingdomBateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United KingdomBateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom

关键词: granulocytes;    inflammation;    chemotaxis;    infection;    phagocytosis;   

DOI  :  10.1189/jlb.3MA0315-105R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.

【 授权许可】

Unknown