Distinct functional roles of amphibian (Xenopus laevis) colony-stimulating factor-1- and interleukin-34-derived macrophages

Journal of Leukocyte Biology

Leon Grayfer¹  Jacques Robert¹ 

[1] Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USADepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USADepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA

关键词: antimicrobial response;    CSF-1;    IL-34;    ranavirus;    myelopoiesis;   

DOI  :  10.1189/jlb.4AB0315-117RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Although MÏ• represent the most primordial immune cell subsets, the mechanisms governing their functional heterogeneity remain poorly defined. However, it is well established that the CSF-1 cytokine contributes to monopoiesis and to this heterogeneity, whereas the unrelated IL-34 also binds the CSF-1R toward poorly understood immunologic roles. To delineate the molecular and evolutionary basis behind vertebrate MÏ• functional heterogeneity, we performed comprehensive transcriptional and functional studies of amphibian (Xenopus laevis) BM (in vitro) and PER (in vivo) MÏ• derived by rXlCSF-1 and rXlIL-34. Our findings indicate that these amphibian cytokines promote morphologically and functionally distinct MÏ• populations. MÏ• induced by rXlCSF-1 possess more robust iNOS gene expression, are substantially more phagocytic, display greater NO responses, and exhibit enhanced bactericidal capacities. By contrast, rXlIL-34-derived MÏ• express greater levels of Arg-1 and NADPH oxidase components and possess greater respiratory burst responses. Most notably, whereas CSF-1 MÏ• are highly susceptible to the emerging FV3 ranavirus, rXlIL-34 MÏ• exhibit potent antiviral activity against this pathogen, which is dependent on reactive oxygen production. This work marks an advance in our understanding of the possible mechanisms governing vertebrate MÏ• functional heterogeneity.

【 授权许可】

Unknown