Distinct functional roles of amphibian (Xenopus laevis) colony‐stimulating factor‐1‐ and interleukin‐34‐derived macrophages

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: antimicrobial response;    CSF‐;    1;    IL‐;    34;    ranavirus;    myelopoiesis;   

DOI  :  10.1189/jlb.4AB0315-117RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

AlthoughMϕrepresentthemostprimordialimmunecellsubsets,themechanismsgoverningtheirfunctionalheterogeneityremainpoorlydefined.However,itiswellestablishedthattheCSF‐1cytokinecontributestomonopoiesisandtothisheterogeneity,whereastheunrelatedIL‐34alsobindstheCSF‐1Rtowardpoorlyunderstoodimmunologicroles.TodelineatethemolecularandevolutionarybasisbehindvertebrateMϕfunctionalheterogeneity,weperformedcomprehensivetranscriptionalandfunctionalstudiesofamphibian(Xenopuslaevis)BM(invitro)andPER(invivo)MϕderivedbyrXlCSF‐1andrXlIL‐34.OurfindingsindicatethattheseamphibiancytokinespromotemorphologicallyandfunctionallydistinctMϕpopulations.MϕinducedbyrXlCSF‐1possessmorerobustiNOSgeneexpression,aresubstantiallymorephagocytic,displaygreaterNOresponses,andexhibitenhancedbactericidalcapacities.Bycontrast,rXlIL‐34‐derivedMϕexpressgreaterlevelsofArg‐1andNADPHoxidasecomponentsandpossessgreaterrespiratoryburstresponses.Mostnotably,whereasCSF‐1MϕarehighlysusceptibletotheemergingFV3ranavirus,rXlIL‐34Mϕexhibitpotentantiviralactivityagainstthispathogen,whichisdependentonreactiveoxygenproduction.ThisworkmarksanadvanceinourunderstandingofthepossiblemechanismsgoverningvertebrateMϕfunctionalheterogeneity...

【 授权许可】

CC BY