The roles of complement receptor 3 and Fcγ receptors during Leishmania phagosome maturation

Journal of Leukocyte Biology

Christopher L. Jerde²  James P. Whitcomb²  Upasna Gaur Dixit³  Rachel Polando²  Mary E. Wilson  and¹  Mary Ann McDowell²  Cristina R. Carter²  Blake Jones²  Wibke Ballhorn²  Melissa Harintho² 

[1] Departments of  Internal Medicine and Microbiology and Epidemiology, University of Iowa and the Veterans Affairs Medical Center, Iowa City, Iowa, USA Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA;Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA;Departments of  Internal Medicine and Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, USA;

关键词: CD11b;    mannose receptor;    deficient;    LAMP1;    EEA1;   

DOI  :  10.1189/jlb.0212086

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Leishmania are intracellular parasites adapted to surviving in macrophages, whose primary function is elimination of invading pathogens. Leishmania entry into host cells is receptor-mediated. These parasites are able to engage multiple host cell-surface receptors, including MR, TLRs, CR3, and FcγRs. Here, we investigated the role of CR3 and FcγR engagement on the maturation of Leishmania-containing phagosomes using CD11b−/− and FcγR−/− macrophages, and assessing EEA1 and lysosome-associated proteins is necessary for the phagosome maturation delay, characteristic of Leishmania infection. Leishmania-containing phagosomes do not fuse with lyosomes until 5 h postinfection in WT mice. Phagolysosome fusion occurs by 1 h in CD11b and FcγR common chain KO macrophages, although receptor deficiency does not influence Leishmania entry or viability. We also investigated the influence of serum components and their effects on phagosome maturation progression. Opsonization with normal mouse serum, complement-deficient serum, or serum from Leishmania-infected mice all influenced phagosome maturation progression. Our results indicate that opsonophagocytosis influences phagosomal trafficking of Leishmania without altering the intracellular fate.

【 授权许可】

Unknown