The roles of complement receptor 3 and Fcγ receptors during Leishmania phagosome maturation

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: CD11b;    mannose receptor;    deficient;    LAMP1;    EEA1;   

DOI  :  10.1189/jlb.0212086

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Leishmaniaareintracellularparasitesadaptedtosurvivinginmacrophages,whoseprimaryfunctioniseliminationofinvadingpathogens.Leishmaniaentryintohostcellsisreceptor‐mediated.Theseparasitesareabletoengagemultiplehostcell‐surfacereceptors,includingMR,TLRs,CR3,andFcγRs.Here,weinvestigatedtheroleofCR3andFcγRengagementonthematurationofLeishmania‐containingphagosomesusingCD11b−/−andFcγR−/−macrophages,andassessingEEA1andlysosome‐associatedproteinsisnecessaryforthephagosomematurationdelay,characteristicofLeishmaniainfection.Leishmania‐containingphagosomesdonotfusewithlyosomesuntil5hpostinfectioninWTmice.Phagolysosomefusionoccursby1hinCD11bandFcγRcommonchainKOmacrophages,althoughreceptordeficiencydoesnotinfluenceLeishmaniaentryorviability.Wealsoinvestigatedtheinfluenceofserumcomponentsandtheireffectsonphagosomematurationprogression.Opsonizationwithnormalmouseserum,complement‐deficientserum,orserumfromLeishmania‐infectedmiceallinfluencedphagosomematurationprogression.OurresultsindicatethatopsonophagocytosisinfluencesphagosomaltraffickingofLeishmaniawithoutalteringtheintracellularfate...

【 授权许可】

CC BY