Viral dsRNA-activated human dendritic cells produce IL-27, which selectively promotes cytotoxicity in naïve CD8+ T cells

Journal of Leukocyte Biology

Astrid J. van Beelen¹  Esther W. M. Taanman-Kueter¹  Ghaith Bakdash¹  Martien L. Kapsenberg¹  Esther C. de Jong¹  Rosa de Groot¹ 

[1] Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, The Netherlands

关键词: BDCA1+ DC;    human cytokines;    Granzyme B;    IFN-gamma;    Cytotoxic T Lymphocytes;   

DOI  :  10.1189/jlb.0112045

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Viral recognition programs DCs to express Signal 3 molecules that promote the differentiation of effector CD8+ T cells. Besides IL-12, another DC-derived IL-12 family member, IL-27, has been reported to contribute herein, but its specific role is not well understood. Here, we show that whereas IL-12 potently induces inflammatory cytokines (i.e., IFN-γ and TNF-α, but not IL-2), IL-27 excels in inducing proliferation and a cytotoxic profile (GrB, cytotoxicity of target cells) in human naïve CD8+ T cells. Compared with bacterial cell-wall peptidoglycan, viral dsRNA-mimic poly (I:C) is superior in priming human BDCA1+ peripheral blood DCs to produce IL-12 and IL-27, which promote inflammatory cytokines and a cytotoxic profile in differentiating CD8+ T cells, respectively. These data support the concept that viral dsRNA-activated human DCs produce IL-27 to act as a specialized procytotoxic, antiviral cytokine in the development of effector CD8+ T cells.

【 授权许可】

Unknown