Effects of palmitoylethanolamide on intestinal injury and inflammation caused by ischemia-reperfusion in mice

Journal of Leukocyte Biology

Emanuela Mazzon⁴  Caterina Faggio²  Francesca Trischitta  and²  Salvatore Cuzzocrea,–¹  Emanuela Esposito⁵  Agata Torre²  Rosanna Di Paola⁵  Daniela Impellizzeri⁵  Alessandro Cappellani³ 

[1] Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, Messina, Italy; –University of Manchester, Manchester, United Kingdom Department of Life Sciences œM. Malpighi, Section of General Physiology and Pharmacology, University of Messina, Italy;Department of Surgery, University of Catania, Italy;Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, Messina, Italy; IRCCS Centro Neurolesi œBonino-Pulejo, Messina, Italy;Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, Messina, Italy;

关键词: circulatory shock;    fatty acid ethanolamide family;    cytokines;    adhesion molecules;    apoptosis;   

DOI  :  10.1189/jlb.0911485

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Our primary aim in this study was to test the hypothesis that PEA, a member of the fatty acid ethanolamide family and an endogenous PPAR-α ligand, exerts anti-inflammatory effects on SAO shock, causing a severe form of circulatory shock and enhanced formation of ROS. SAO shock was induced by clamping the superior mesenteric artery and the celiac trunk, resulting in a total occlusion of these arteries for 30 min. After this period of occlusion, the clamps were removed. In this study, we demonstrated that the administration of PEA, 5 min before reperfusion, significantly reduced all of the parameters involved during inflammation, such as proinflammatory cytokine production (TNF-α, IL-1β), adhesion molecules (ICAM-1, P-selectin) expression, NF-κB expression, and apoptosis (Bax, Bcl-2, TUNEL assay) activation. In addition, to study whether the protective action of PEA on SAO shock is also related to the activation of PPAR-α, we have investigated the effect of PEA in PPAR-α KO mice subjected to SAO shock. Our study clearly demonstrates that PEA significantly attenuated the degree of intestinal injury and inflammation caused by I/R injury. Moreover, the positive effects of PEA were at least in part dependent on the PPAR-α pathway. The results clearly indicate that PEA exerts an anti-inflammatory effect, also in a SAO shock model, which could imply a future use of PEA in the treatment of I/R shock.

【 授权许可】

Unknown