Effects of palmitoylethanolamide on intestinal injury and inflammation caused by ischemia‐reperfusion in mice

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: circulatory shock;    fatty acid ethanolamide family;    cytokines;    adhesion molecules;    apoptosis;   

DOI  :  10.1189/jlb.0911485

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

OurprimaryaiminthisstudywastotestthehypothesisthatPEA,amemberofthefattyacidethanolamidefamilyandanendogenousPPAR‐αligand,exertsanti‐inflammatoryeffectsonSAOshock,causingasevereformofcirculatoryshockandenhancedformationofROS.SAOshockwasinducedbyclampingthesuperiormesentericarteryandtheceliactrunk,resultinginatotalocclusionofthesearteriesfor30min.Afterthisperiodofocclusion,theclampswereremoved.Inthisstudy,wedemonstratedthattheadministrationofPEA,5minbeforereperfusion,significantlyreducedalloftheparametersinvolvedduringinflammation,suchasproinflammatorycytokineproduction(TNF‐α,IL‐1β),adhesionmolecules(ICAM‐1,P‐selectin)expression,NF‐κBexpression,andapoptosis(Bax,Bcl‐2,TUNELassay)activation.Inaddition,tostudywhethertheprotectiveactionofPEAonSAOshockisalsorelatedtotheactivationofPPAR‐α,wehaveinvestigatedtheeffectofPEAinPPAR‐αKOmicesubjectedtoSAOshock.OurstudyclearlydemonstratesthatPEAsignificantlyattenuatedthedegreeofintestinalinjuryandinflammationcausedbyI/Rinjury.Moreover,thepositiveeffectsofPEAwereatleastinpartdependentonthePPAR‐αpathway.TheresultsclearlyindicatethatPEAexertsananti‐inflammatoryeffect,alsoinaSAOshockmodel,whichcouldimplyafutureuseofPEAinthetreatmentofI/Rshock...

【 授权许可】

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