Expansion of a subset of CD14highCD16negCCR2low/neg monocytes functionally similar to myeloid-derived suppressor cells during SIV and HIV infection

Journal of Leukocyte Biology

Julia N. Russell²  Jorge Kalil¹  Ming Li²  M. Christine Zink²  Lucio Gama, ³  Karina I. Carvalho¹  Erin N. Shirk²  Suzanne E. Queen²  Janice E. Clements and²  Esper G. Kallas ¹ 

[1]  Division of Clinical Immunology and Allergy, University of São Paulo School of Medicine, São Paulo, Brazil Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;;Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; and  Division of Clinical Immunology and Allergy, University of São Paulo School of Medicine, São Paulo, Brazil

关键词: macrophages;    AIDS;   

DOI  :  10.1189/jlb.1111579

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Monocytes have been categorized in three main subpopulations based on CD14 and CD16 surface expression. Classical monocytes express the CD14++CD16−CCR2+ phenotype and migrate to inflammatory sites by quickly responding to CCL2 signaling. Here, we identified and characterized the expansion of a novel monocyte subset during HIV and SIV infection, which were undistinguishable from classical monocytes, based on CD14 and CD16 expression, but expressed significantly lower surface CCR2. Transcriptome analysis of sorted cells demonstrated that the CCR2low/neg cells are a distinct subpopulation and express lower levels of inflammatory cytokines and activation markers than their CCR2high counterparts. They exhibited impaired phagocytosis and greatly diminished chemotaxis in response to CCL2 and CCL7. In addition, these monocytes are refractory to SIV infection and suppress CD8+ T cell proliferation in vitro. These cells express higher levels of STAT3 and NOS2, suggesting a phenotype similar to monocytic myeloid-derived cells, which suppress expansion of CD8+ T cells in vivo. They may reflect an antiproliferative response against the extreme immune activation observed during HIV and SIV infections. In addition, they may suppress antiviral responses and thus, have a role in AIDS pathogenesis. Antiretroviral therapy in infected macaque and human subjects caused this population to decline, suggesting that this atypical phenotype is linked to viral replication.

【 授权许可】

Unknown