Human CD16+ and CD16– monocyte subsets display unique effector properties in inflammatory conditions in vivo

Journal of Leukocyte Biology

Gabriela GonzÃlez-Pérez¹  Eduardo A. García-Zepeda and²  Carmen SÃnchez-Torres¹  Honorio Torres-Aguilar¹  Érika GonzÃlez-Domínguez¹  GermÃn Vargas-Ayala³  Marco A. Meraz-Ríos¹  Sergio R. Aguilar-Ruiz¹  Juana NarvÃez¹ 

[1] Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, Mexico;Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, Mexico; Department of Internal Medicine, Ticoman General Hospital, Mexico City, Mexico;

关键词: myeloid differentiation;    TLR ligands;   

DOI  :  10.1189/jlb.0111022

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Two major subsets of human Mo are identified based on CD14 and CD16 expression: the classical CD16– Mo and the minor CD14+CD16+ Mo. In vitro studies suggested distinct function and differentiation potential for each cell population. However, the in vivo relevance of these findings remains unclear. To evaluate the development and function of human Mo in an in vivo model, we transferred both Mo subpopulations into the peritoneum of immunocompromised mice in homeostatic or inflammatory conditions. Inflammation was induced with soluble LPS or particulate zymosan. CD16+ were more phagocytic and produced higher amounts of TNF and IL-6 than CD16– Mo early after transfer with zymosan. They also produced higher levels of β2-defensin in any condition evaluated, which could represent a new marker for this subpopulation. In contrast, differentiating CD16– Mo (24 h after transfer) acquired greater APC capacity in LPS-induced peritonitis, whereas none of the Mo subsets attained this ability with zymosan. CX3CL1 supported the survival of both Mo subsets in vivo. Similar Mo subpopulations were present in human peritonitis. These results support the idea of specialized roles of the Mo subset, where CD16+ might act in an immediate innate immune response, whereas CD16– could have a major role as APCs.

【 授权许可】

Unknown