Human CD16+ and CD16– monocyte subsets display unique effector properties in inflammatory conditions in vivo

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: myeloid differentiation;    TLR ligands;   

DOI  :  10.1189/jlb.0111022

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TwomajorsubsetsofhumanMoareidentifiedbasedonCD14andCD16expression:theclassicalCD16–MoandtheminorCD14+CD16+Mo.Invitrostudiessuggesteddistinctfunctionanddifferentiationpotentialforeachcellpopulation.However,theinvivorelevanceofthesefindingsremainsunclear.ToevaluatethedevelopmentandfunctionofhumanMoinaninvivomodel,wetransferredbothMosubpopulationsintotheperitoneumofimmunocompromisedmiceinhomeostaticorinflammatoryconditions.InflammationwasinducedwithsolubleLPSorparticulatezymosan.CD16+weremorephagocyticandproducedhigheramountsofTNFandIL‐6thanCD16–Moearlyaftertransferwithzymosan.Theyalsoproducedhigherlevelsofβ2‐defensininanyconditionevaluated,whichcouldrepresentanewmarkerforthissubpopulation.Incontrast,differentiatingCD16–Mo(24haftertransfer)acquiredgreaterAPCcapacityinLPS‐inducedperitonitis,whereasnoneoftheMosubsetsattainedthisabilitywithzymosan.CX3CL1supportedthesurvivalofbothMosubsetsinvivo.SimilarMosubpopulationswerepresentinhumanperitonitis.TheseresultssupporttheideaofspecializedrolesoftheMosubset,whereCD16+mightactinanimmediateinnateimmuneresponse,whereasCD16–couldhaveamajorroleasAPCs...

【 授权许可】

CC BY