Expression of C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages, and monocytes

Journal of Leukocyte Biology

Koji Nagaoka²  Yoko Yoshida and²  Kento Minamino²  Tatsuki Takeda²  Kayo Inaba ¹  Kazuhiko Takahara¹ 

[1] Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; and  JST, CREST, Yoshida-Konoe, Sakyo, Kyoto, JapanDepartment of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan;

关键词: rodent;    cell differentiation;    skin;    spleen;    lymph nodes;    lung;   

DOI  :  10.1189/jlb.0510251

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The C-type lectin SIGNR3 is a mouse homologue of human DC-SIGN, which shares carbohydrate-binding specificity with human DC-SIGN. However, the expression profile of SIGNR3 is largely unknown. To examine the expression of SIGNR3 in immune cells, we generated SIGNR3-specific mAb and investigated SIGNR3 expression in vivo. SIGNR3 was expressed on a fraction of MHC II+ DCs and Mφs in the dermis and CD115+Ly6Cint-low monocytes in the blood and BM. In the LNs, SIGNR3+ cells localized adjacent to PNAd+ HEV-like vessels. They were also found in interfollicular regions in sLNs but not mLNs. Those SIGNR3+ cells expressed CD11b and variable levels of CD11c and MHC II. As in LNs, SIGNR3 was expressed on a large proportion of the CD11b+CD11cint-high cells in the spleen. In the lung, SIGNR3+ cells belonged to the CD11b+CD11cint population, and Mφs in the airway and lung faintly expressed SIGNR3. When PKH67-labeled CD115+Ly6Chigh BM monocytes were transferred into normal recipients, they up-regulated SIGNR3 expression along with the decrease in Ly6C expression during the circulation and upon arrival at the peripheral LNs through HEV. In addition, CD11bhighLy6Chigh monocytes that entered sLNs differentiated into CD11b+ DCs in a couple of days, whereas those in the spleen, mLNs, and lung differentiated into CD11cint monocytic cells. These results suggest that SIGNR3 is a new differentiation marker for myeloid mononuclear cells and indicate that some DCs, especially in the sLNs, are possibly replenished by Ly6Chigh monocytes.

【 授权许可】

Unknown