Involvement of CCR5 in the passage of Th1-type cells across the blood-retina barrier in experimental autoimmune uveitis

Journal of Leukocyte Biology

Heping Xu³  Isabel J. Crane³  Gabriel Marquez²  Ayyakkannu Manivannan¹  Peter F. Sharp¹  Carol Wallace³  John V. Forrester³  Janet Liversidge³  Matthias Mack⁴ 

[1] Bio-Medical Physics and Bio-Engineering, University of Aberdeen Medical School, Foresterhill, United Kingdom; Bio-Medical Physics and Bio-Engineering, University of Aberdeen Medical School, Foresterhill, United Kingdom; Bio-Medical Physics and Bio-Engineering, University of Aberdeen Medical School, Foresterhill, United Kingdom;;Centro Nacional de Biotecnologia, UAM-Campus de Cantoblanco, Madrid, Spain Centro Nacional de Biotecnologia, UAM-Campus de Cantoblanco, Madrid, Spain Centro Nacional de Biotecnologia, UAM-Campus de Cantoblanco, Madrid, Spain;Ophthalmology and Ophthalmology and Ophthalmology and;Department of Internal Medicine II, University of Regensburg, Germany; and Department of Internal Medicine II, University of Regensburg, Germany; and Department of Internal Medicine II, University of Regensburg, Germany; and

关键词: chemokines;    chemokine receptor;    cell trafficking;    inflammation;   

DOI  :  10.1189/jlb.0305130

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Although the recruitment of T helper cell type 1 (Th1)/Th2 cells into peripheral tissues is essential for inflammation and the host response to infection, the traffic signals that enable the distinct positioning of Th1/Th2 cells are unclear. We have determined the role of CC chemokine receptor 5 (CCR5) in this using experimental autoimmune uveitis (EAU) as a model system. In EAU, Th1-like cells are preferentially recruited into the retina across the blood-retina barrier, partly as a result of expression of the adhesion molecules P-selectin glycoprotein ligand 1 and lymphocyte function-associated antigen-1 on these cells. CD3+ T cells, infiltrating the retina, also expressed the chemokine receptor CCR5, and CCR5 ligands, macrophage-inflammatory protein-1α (MIP-1α), MIP-1β, and regulated on activation, normal T expressed and secreted (RANTES), were strongly expressed in the retina at peak EAU. Th1-like cells, polarized in vitro, expressed high levels of CCR5. The trafficking of these CCR5+ cells was examined by tracking them after adoptive transfer in real time in vivo at an early disease stage using scanning laser ophthalmoscopy. Treatment of the cells with antibody against CCR5 prior to transfer resulted in a reduction in their infiltration into the retina. However, rolling velocity, rolling efficiency, and adherence of the cells to retinal endothelium were not reduced. CCR5 is clearly important for Th1 cell recruitment, and this study demonstrates for the first time in vivo that CCR5 may act at the level of transendothelial migration rather than at the earlier stage of rolling on the endothelium.

【 授权许可】

Unknown