Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS

Journal of Leukocyte Biology

Giovanna Borsellino⁴  Antonio Uccelli³  Giorgio Bernardi⁷  Roberto Benelli²  Enrico Pedemonte⁶  Maria Teresa Valle¹  Elisabetta Capello³  Luca Battistini⁴  Monica Marchese⁶  Debora Giunti⁶  Adriana Albini²  Giovanni Luigi Mancardi³  Douglas Noonan⁵ 

[1] II Division of Haematology, S. Martino Hospital, Genoa, Italy; and II Division of Haematology, S. Martino Hospital, Genoa, Italy; and II Division of Haematology, S. Martino Hospital, Genoa, Italy; and;Molecular Biology Laboratory and Molecular Biology Laboratory and Molecular Biology Laboratory andNeuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Centre of Excellence for Biomedical Research, University of Genoa, Italy; Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Centre of Excellence for Biomedical Research, University of Genoa, Italy; Centre of Excellence for Biomedical Research, University of Genoa, Italy; Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Centre of Excellence for Biomedical Research, University of Genoa, Italy;Laboratory of Neuroimmunology, IRCCS, S. Lucia, Rome, Italy; Laboratory of Neuroimmunology, IRCCS, S. Lucia, Rome, Italy; Laboratory of Neuroimmunology, IRCCS, S. Lucia, Rome, Italy;#Tumor Progression Section, National Cancer Research Institute, Genoa, Italy; #Tumor Progression Section, National Cancer Research Institute, Genoa, Italy; #Tumor Progression Section, National Cancer Research Institute, Genoa, Italy;;Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and;Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Department of Neuroscience, University of Rome “Tor Vergata,” Italy Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Department of Neuroscience, University of Rome “Tor Vergata,” Italy Department of Neuroscience, University of Rome “Tor Vergata,” Italy Neuroimmunology Unit, Department of Neurosciences, Ophthalmology and Genetics, and Department of Neuroscience, University of Rome “Tor Vergata,” Italy

关键词: MS;    chemokines;    T lymphocytes;    neuroimmunology;    cell trafficking;   

DOI  :  10.1189/jlb.1202598

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The recruitment of lymphocytes across the blood brain barrier (BBB) is mediated by adhesion molecules and chemokines. The expression of activation markers and of chemokine receptors on T cells homing to the nervous system (NS) may help define their functional state. In the cerebrospinal fluid (CSF) of subjects with inflammatory neurological diseases (IND), including multiple sclerosis, we observed an increased number of T cells coexpressing CXCR3 and CCR5 as well as T cells with a CD45RO+ CCR7+ CD27+ memory phenotype. A subset of CCR7+ T cells coexpressed CXCR3 and CCR5. We also detected an increased number of interferon-γ-producing T cells in the CSF compared with peripheral blood, mostly but not exclusively in the CD45RO+ CCR7− CD27− compartment. T helper 1 (Th1) clones, established from the CSF of individuals with IND and from a healthy subject, similarly migrated to CXCL10, CXCL12, and CCL5. CXCL10, CXCL12, and CCL19 were increased in the CSF of individuals with neuroinflammation. These findings suggest that CSF is enriched in Th1-polarized memory T cells capable of differentiating into effector cells upon antigen encounter. These cells are recruited into the CSF by inducible chemokines. Thus, CSF represents a transitional station for T cells trafficking to and from the NS.

【 授权许可】

Unknown