Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration

Journal of Leukocyte Biology

Ewan A. Ross²  Oliver Haworth²  Gerard B. Nash²  Chloe. E. Osborne²  Mike Salmon²  Christopher D. Buckley²  Helen M. McGettrick²  Philip C. W. Stone²  Rajiv K. Vohra¹  Caroline Schmutz²  G. Ed Rainger²  Nick M. Matharu² 

[1] Department of Vascular Surgery, University of Birmingham Hospital (Selly Oak), United Kingdom Department of Vascular Surgery, University of Birmingham Hospital (Selly Oak), United Kingdom Department of Vascular Surgery, University of Birmingham Hospital (Selly Oak), United Kingdom;The Centre for Cardiovascular Sciences, Centre for Immune Regulation and the MRC Centre, The University of Birmingham, United Kingdom; and The Centre for Cardiovascular Sciences, Centre for Immune Regulation and the MRC Centre, The University of Birmingham, United Kingdom; and The Centre for Cardiovascular Sciences, Centre for Immune Regulation and the MRC Centre, The University of Birmingham, United Kingdom; and

关键词: migration;    recirculation;    chronic inflammation;   

DOI  :  10.1189/jlb.0905496

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Recent studies have demonstrated that neutrophils are not a homogenous population of cells. Here, we have identified a subset of human neutrophils with a distinct profile of cell-surface receptors [CD54high, CXC chemokine receptor 1low (CXCR1low)], which represent cells that have migrated through an endothelial monolayer and then re-emerged by reverse transmigration (RT). RT neutrophils, when in contact with endothelium, were rescued from apoptosis, demonstrate functional priming, and were rheologically distinct from neutrophils that had not undergone transendothelial migration. In vivo, 1–2% of peripheral blood neutrophils in patients with systemic inflammation exhibit a RT phenotype. A smaller population existed in healthy donors (≈0.25%). RT neutrophils were distinct from naïve circulatory neutrophils (CD54low, CXCR1high) and naïve cells after activation with formyl-Met-Leu-Phe (CD54low, CXCR1low). It is important that the RT phenotype (CD54high, CXCR1low) is also distinct from tissue-resident neutrophils (CD54low, CXCR1low). Our results demonstrate that neutrophils can migrate in a retrograde direction across endothelial cells and suggest that a population of tissue-experienced neutrophils with a distinct phenotype and function are present in the peripheral circulation in humans in vivo.

【 授权许可】

Unknown