Interleukin-15 enhances human neutrophil phagocytosis by a Syk-dependent mechanism: importance of the IL-15Rα chain

Journal of Leukocyte Biology

Denis Girard¹  Claude Ratthé¹ 

[1] INRS-Institut Armand-Frappier, Université du Québec, Pointe-Claire, CanadaINRS-Institut Armand-Frappier, Université du Québec, Pointe-Claire, CanadaINRS-Institut Armand-Frappier, Université du Québec, Pointe-Claire, Canada

关键词: inflammation;    cytokine receptor;    tyrosine kinases;    CD122;    CD132;   

DOI  :  10.1189/jlb.0605298

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Interleukin-15 (IL-15) is a cytokine that possesses interesting, potential therapeutic properties. However, based on several parameters including activation of neutrophils, it is also recognized as a proinflammatory cytokine. The mechanisms by which IL-15 activates human neutrophil functions are not fully understood. Although these cells express a functional IL-15 receptor (IL-15R) composed of IL-15Rα, IL-2/15Rβ (CD122), and γc (CD132) subunits, the role of each receptor component has not been investigated in IL-15-induced human neutrophil responses. In the present study, fluorescein-activated cell sorter analysis revealed that the ability of IL-15 to enhance neutrophil phagocytosis is not a result of increased expression of IL-15Rα, CD122, or CD132 on the neutrophil cell surface. Pretreatment of neutrophils with specific antibodies to IL-15Rα, CD122, or CD132 was found to inhibit phagocytosis of opsonized-sheep red blood cells by nearly 40%, 21%, and 27%, respectively. As expected, pretreatment of neutrophils with anti-IL-2Rα (CD25) had no effect. Pretreatment of cells with the Syk inhibitor piceatannol was found to significantly inhibit the ability of IL-15 to enhance phagocytosis. In addition, IL-15 was found to induce tyrosine phosphorylation of Syk that was largely inhibited by pretreating cells with piceatannol. Moreover, we found that Syk kinase is physically associated with IL-15Rα. We conclude that IL-15R enhances neutrophil phagocytosis by a Syk-dependent mechanism and that the IL-15Rα chain plays a key role in mediating this response, at least by interacting with Syk kinase.

【 授权许可】

Unknown