Changes in peritoneal myeloid populations and their proinflammatory cytokine expression during infection with Listeria monocytogenes are altered in the absence of γ/δ T cells

Journal of Leukocyte Biology

H. Kirk Ziegler¹  Molly M. Freeman¹  Marianne J. Skeen¹ 

[1] Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GeorgiaDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GeorgiaDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia

关键词: macrophages;    monocytes;    neutrophils;    TNF;    IL-12;   

DOI  :  10.1189/jlb.1103574

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Evidence that γ/δ T cells play a broad, immunoregulatory role has been accumulating steadily. We show here that myeloid cells are disregulated after peritoneal infection with Listeria monocytogenes in mice lacking γ/δ T cells. Inflammatory populations of neutrophils and monocytes recruited to the site of infection remained longer. Intracellular cytokine analysis showed that frequencies of myeloid cells producing interleukin-12 and tumor necrosis factor α were higher and remained elevated longer after infection in mice genetically deficient in γ/δ T cells. In vivo dye-tracking studies indicated that the majority of inflammatory monocytes differentiated into resident tissue macrophages in situ. In vitro experiments confirmed that monocytes harvested from mice lacking γ/δ T cells were defective in their maturation process. This evidence suggests that γ/δ T cells promote differentiation in the monocyte/macrophage lineage. These cells are important for bactericidal activity, inflammatory cytokine production, clearance of inflammatory neutrophils, and ultimately, antigen presentation to T cells. Regulation of monocyte/macrophage differentiation may underlie a broad segment of the phenotypic alterations that have been reported in mice lacking γ/δ T cells.

【 授权许可】

Unknown