Studying the Innate Immune Response to Myocardial Infarction in a Highly Efficient Experimental Animal Model

Romanian Journal of Cardiology

Doina MANU¹  Mihaela Elena TOMUT²  Alexandru SCHIOPU³  Gabriel JAKOBSSON³  Gabriela PINTICAN⁴  Razvan Gheorghita MARES⁵  Ovidiu Simion COTOI⁵  Bogdan CORDOS⁵  Minodora DOBREANU⁵  Istvan Adorjan SZABO⁵ 

[1] Center for Advanced Medical and Pharmaceutical Research, Targu Mures, Romania;Clinical County Hospital, Targu Mures, Romania;Department of Clinical Sciences Malmö, Lund University, Sweden;Emergency Clinical County Hospital, Targu Mures, Romania;„George Emil Palade” University of Medicine, Pharmacy, Science and Technology, Targu Mures, Romania;

关键词: acute myocardial infarction;    experimental model;    innate immunity;    neutrophils;    monocytes;    macrophages;    myocardial fibrosis;    revascularization;   

DOI  :  https://doi.org/10.47803/rjc.2021.31.3.573

来源: DOAJ

【 摘 要 】

The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has at tracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary artery (LAD) in mice, followed by complex flow-cytometry and histological analyses of immune cellular populations in blood and myocardium. We are presenting time-dependent qualitative and quantitative analysis results, demonstrating intense accumulation of Ly6Ghi neutrophils and Ly6Chi monocytes in the infarcted myocardium on days 1 and 3 post-AMI, followed by successive accumulation of reparatory Ly6CloMerTKhi macrophages, neovascularization and fibrosis development by day 7.

【 授权许可】

Unknown