Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation

Journal of Leukocyte Biology

M. A. P. Oliveira¹  G. M. A. C. Lima²  P. J. M. Leenen³  I. A. Abrahamsohn²  M. T. Shio² 

[1] Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Present address: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Present address: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil Present address: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Present address: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, BrasilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil; Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil;Department of Immunology, Erasmus MC, Rotterdam, The Netherlands; Department of Immunology, Erasmus MC, Rotterdam, The Netherlands; Department of Immunology, Erasmus MC, Rotterdam, The Netherlands;

关键词: monocyte/macrophage differentiation;    IL-12;    nitric oxide;    M-CSF;    GM-CSF;    IL-3;   

DOI  :  10.1189/jlb0302124

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Production of IL-12 is an important indicator of the macrophage’s ability to regulate immune responses. In this study, we investigated the IL-12 production by macrophages in different developmental stages. To this end, macrophages were generated in vitro from precursors stimulated with M-CSF, GM-CSF or IL-3. Density separation yielded populations enriched in different maturation stages. Invariably, only cells banding at the 40−50% Percoll interface produced large amounts of IL-12p40 when stimulated with LPS, whereas only low levels of IL-12p70 were produced. These cells represented immature macrophages, as indicated by the absence of precursor markers CD31/ER-MP12, Ly-6C/ER-MP20 and ER-MP58, and by the low level of expression of mature-cell markers like ER-HR3, scavenger receptor and CD11b/Mac-1. Upon further maturation, the macrophages’ ability to produce IL-12p40 decreased, coinciding with increased nitric oxide production upon LPS stimulation. These results show that immature macrophages produce high levels of IL-12p40 and thus may either contribute to IL-12p70 production or regulate it.

【 授权许可】

Unknown