IgD-receptor (IgD-R) cross-linking partially protects murine T cells from dexamethasone-induced apoptosis

Journal of Leukocyte Biology

Seetha M. Lakshmi Tamma²  Richard F. Coico¹ 

[1] Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and;Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Biomedical Sciences, C. W. Post, Long Island University, Brookville, New York Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Biomedical Sciences, C. W. Post, Long Island University, Brookville, New York Department of Biomedical Sciences, C. W. Post, Long Island University, Brookville, New York Department of Microbiology and Immunology, CUNY Medical School, New York, New York; and Department of Biomedical Sciences, C. W. Post, Long Island University, Brookville, New York

关键词: IgD;    T cell activation;    Fas;   

DOI  :  10.1189/jlb.1002492

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Based on our previous findings that immunoglobulin D (IgD) receptor (IgD-R) cross-linking with oligomeric IgD (IgD-R-xL) led to T cell activation, we examined the effect of IgD-R-xL on the expression of Fas antigen and apoptosis induction. In splenic T cells, IgD-R-xL followed by dexamethasone (dex) treatment resulted in a decreased percentage of Fas-positive cells as well as a decreased mean fluorescence intensity (P<0.05) when compared with cells treated with dex alone. There are significant differences in annexin–fluorescein isothiocyanate (FITC) and phosphatidylinositol (PI) staining between samples treated with dex alone and IgD-R-xL followed by dex-treated samples (P<0.05), suggesting a protective role for IgD-R-xL. No significant differences are seen in Fas antigen expression, annexin–FITC staining, and/or PI staining in murine T hybridoma (7C5) cells cultured under similar conditions (P<0.07). We hypothesize that ligation of IgD-R may predispose antigen-specific T lymphocytes for survival during primary immune responses when IgD-positive B cells serve as antigen-presenting cells.

【 授权许可】

Unknown