Imatinib restores expression of CD62L in BCR-ABL-positive cells

Journal of Leukocyte Biology

A. D. Ho²  A. Hochhaus¹  H. Gschaidmeier³  J. Topaly²  M. Schad⁴  W. J. Zeller⁴  P. Paschka¹  S. Fruehauf² 

[1] III, Medizinische Klinik, Fakultät für Klinische Medizin Mannheim, Universität Heidelberg, Mannheim, Germany; and III, Medizinische Klinik, Fakultät für Klinische Medizin Mannheim, Universität Heidelberg, Mannheim, Germany; and III, Medizinische Klinik, Fakultät für Klinische Medizin Mannheim, Universität Heidelberg, Mannheim, Germany; andDepartment of Internal Medicine V, University of Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, Germany;;Novartis Pharma GmbH, Nürnberg, Germany Novartis Pharma GmbH, Nürnberg, Germany Novartis Pharma GmbH, Nürnberg, GermanyD0200, German Cancer Research Center, Heidelberg, Germany; D0200, German Cancer Research Center, Heidelberg, Germany; D0200, German Cancer Research Center, Heidelberg, Germany;

关键词: chronic myelogenous leukemia;    adhesion molecule;    L-selectin;    imatinib mesylate;   

DOI  :  10.1189/jlb.1002507

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Chronic myelogenous leukemia (CML) is characterized by aberrant trafficking of malignant hematopoietic progenitor cells in the peripheral blood. Expression of the cell adhesion molecule CD62L was reported to be significantly lower in CML patients than in normal controls. We studied whether the transcription of CD62L in CML cells is dependent on the activity of the BCR-ABL tyrosine kinase. Following addition of the Abelson (ABL) tyrosine kinase inhibitor imatinib (formerly STI571) to two BCR-ABL-positive cell lines (BV173, SD-1), we observed a dose-dependent increase in CD62L RNA levels of up to 45-fold by a quantitative, real-time polymerase chain reaction and an increase in the amount of cell surface-bound CD62L of up to 18-fold by quantitative flow cytometry, respectively. These data are validated by an increased CD62L expression in the bone marrow of patients (n=6) with advanced CML who received imatinib. Restoration of defective cell adhesion mediated via the CD62L pathway may be one mechanism of action of imatinib in BCR-ABL-positive leukemias.

【 授权许可】

Unknown