Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma

Sensors

Thong M. Cao¹  Michael J. Mitchell¹  Jane Liesveld² 

[1] Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA; E-Mails:;School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA; E-Mail:

关键词: CD34+ hematopoietic stem cells;    L-selectin;    biomimetic;    acidic pH;   

DOI  :  10.3390/s130912516

来源: mdpi

【 摘 要 】

The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.