Sensors | |
Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma | |
Thong M. Cao1  Michael J. Mitchell1  Jane Liesveld2  | |
[1] Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA; E-Mails:;School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA; E-Mail: | |
关键词: CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH; | |
DOI : 10.3390/s130912516 | |
来源: mdpi | |
【 摘 要 】
The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190033003ZK.pdf | 542KB | download |