Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp

Planta Daninha

UFV¹  Tavares, S.S.¹  Assunção, I.P.¹  Ramos-Sobrinho, R.¹  Lima, G.S.A.¹  Universidade Federal de Alagoas, Rio Largo, Brazil¹  Universidade Federal de Viçosa, Viçosa, Brasil¹  González-Aguilera, J.¹  Zerbini, F.M¹ 

关键词: geminivirus;    recombination;    Blainvillea rhomboidea;    Sida micrantha;    Sida urens;    Sida santaremnensis;   

DOI  :  10.1590/S0100-83582012000200009

学科分类：农业科学（综合）

来源: Sociedade Brasileira da Ciencia das Plantas Daninhas (S B C P D) / Brazilian Society of Herb Science

【 摘 要 】

Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants. The aim of this study was to further characterize the diversity of begomoviruses infecting weeds (mostly Sida spp.) in Brazil. Total DNA was extracted from weed samples collected in Viçosa (Minas Gerais state) and in some municipalities of Alagoas state in 2009 and 2010. Viral genomes were amplified by RCA, cloned and sequenced. A total of 26 DNA-A clones were obtained. Sequence analysis indicated the presence of 10 begomoviruses. All viral isolates from Blainvillea rhomboidea belonged to the same species, Blainvillea yellow spot virus (BlYSV ), thereby suggesting that BlYSV may be the only begomovirus present in this weed species. Four isolates represent new species, for which the following names are proposed: Sida yellow blotch virus (SiYBV), Sida yellow net virus (SiYNV), Sida mottle Alagoas virus (SiMoAV) and Sida yellow mosaic Alagoas virus (SiYMAV). Recombination events were detected among the SiYBV isolates and in the SiYNV isolate. These results constitute further evidence of the high species diversity of begomoviruses in Sida spp. However, the role of this weed species as a source of begomoviruses infecting crop plants remains to be determined.

【 授权许可】

Unknown