Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China

Viruses

Xiaoyu Li¹  Xiuling Yang²  Min Du²  Xueping Zhou²  Yongzhi Wang²  Runzi Feng²  Cheng Chen² 

[1] Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Jilin Academy of Agricultural Sciences, Changchun 130033, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;

关键词: geminivirus;    soybean;    recombination;    ssDNA virus;   

DOI  :  10.3390/v14020341

来源: DOAJ

【 摘 要 】

Soybean is a major legume crop that plays an important role in food production, industrial production, and animal husbandry. Here, we characterize a novel soybean-infecting monopartite geminivirus identified in China. Analysis of the contigs de novo assembled from sequenced small interfering RNAs, followed by PCR, cloning, and sequencing, the complete viral genome was determined to be 2782 nucleotides. The genome contains the conserved nonanucleotide sequence, TAATATTAC and other sequence features typical of the family Geminiviridae, and encodes two and four open reading frames in the virion-sense and the complementary-sense strands, respectively. Genome-wide pairwise identity analysis revealed that the novel virus shares less than 65.6% identity with previously characterized geminiviruses. Phylogenetic and recombination analysis indicated that this virus was placed in a unique taxon within the family Geminiviridae and potentially arose from recombination. An infectious clone of this virus was further constructed and its infectivity was tested in different species of plants. Successful infection and characteristic symptoms were observed in Glycine max, Nicotiana benthamiana, N. tabacum, N. glutinosa, and N. tabacum cv. Samsun plants. Taken together, this virus represents a member of an unclassified genus of the family Geminiviridae, for which the name soybean yellow leaf curl virus is proposed.

【 授权许可】

Unknown