Improving tuberculosis control through the partnership between university and the health system

Revista da Sociedade Brasileira de Medicina Tropical

Macedo, Maíra Bidart de¹  Silva, Pedro Eduardo Almeida da¹  von Groll, Andrea¹  Scaini, Carlos James¹  Madruga, Catiúcia¹  Universidade Federal do Rio Grande, Rio Grande¹  Valença, Mariana Soares¹  Carrion, Lillian Lucas¹  Rocha, Jeane Zanini da¹  Ramis, Ivy Bastos¹ 

关键词: Mycobacterium tuberculosis;    Diagnostic;    Molecular epidemiology;   

DOI  :  10.1590/S0037-86822012005000004

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control.METHODS: Direct microscopy of sputum and culture in Ogawa-Kudoh were performed on 1,636 samples from 787 patients. DST of positive cultures was performed by resazurin microtiter assay and genotyping by mycobacterial interspersed repetitive units-variable number tandem repeat.RESULTS: A total 91 patients with TB were identified. The culture increased case detection by 32% compared with the microscopy; acquired resistance was 3.3% and the genotyping showed high genetic diversity.CONCLUSIONS: Ogawa-Kudoh contributed significantly to the increase in case detection and is suitable for implementation in poor-resource locations. The acquired resistance rate was lower than that reported in a recent Brazilian survey. The high genetic diversity is possibly related to the high TB prevalence in the population, as well as to early detection and suitable treatment of patients. The interaction between research and health care is important for reorienting the practice, transferring technology, and improving TB control.

【 授权许可】

Unknown