Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Revista da Sociedade Brasileira de Medicina Tropical

Oliveira, Ligene Fernandes de¹  Santos, Glauco Jonas Lemos¹  Lopes Neto, Belarmino Eugênio¹  Campos, Rafael de Morais¹  Nunes-Pinheiro, Diana Célia Sousa¹  Universidade Federal do Ceará, Fortaleza¹  Universidade Estadual do Ceará, Fortaleza¹  Freitas, José Cláudio Carneiro de¹  Abreu, Cyntia Rafaelle Amaral de¹  Braga, Roberta Rocha¹  Centro de Controle de Zoonoses de Fortaleza, Fortaleza¹ 

关键词: Dogs;    Canine visceral leishmanisis;    Biomarkers;    Anemia;    Uremia;    Hyperglobulinemia;   

DOI  :  10.1590/S0037-86822012000100006

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Canine visceral leishmaniasis (CVL) is a zoonotic disease with different clinical manifestations. Parasitism often occurs in bone marrow, but changes have been observed in peripheral blood and serum biochemical parameters. The aim of this study was to evaluate the hematological and biochemical parameters in dogs naturally infected by Leishmania chagasi.METHODS: Eighty-five adult dogs of both sexes and various weights and ages from the Zoonosis Control Center of Fortaleza (CCZ) were used, selected by immunofluorescence assay (IFA) and considered positive with IFA titers greater than 1:40 and by visualizing amastigotes of Leishmania chagasi in smears obtained by bone marrow aspiration. The dogs (n = 85) were grouped according to clinical signs: negative (CN = 7), subclinical (CS = 10), and clinical (CC = 68). Blood samples were collected for determination of hematological and biochemical serum values. The experimental protocol was approved by the CEUA/UECE.RESULTS: The most frequent clinical signs were cachexia (77.9%), keratitis (61.8%), and lymphadenopathy (55.9%), and 86.8% of the animals showed more than one clinical sign characteristic of CVL. In CC were observed reductions in red blood cells (63%), hematocrit (72%), and hemoglobin (62%), as well as leukocytosis (33%), neutropenia (28%), thrombocytopenia (50%), uremia (45%), hyperproteinemia (53%, p<0.05), hypergammaglobulinemia (62%, p<0.01), and hypoalbuminemia (58%).CONCLUSIONS: Animals with the clinical form of the disease demonstrate hematological and biochemical changes consistent with anemia, uremia, hyperproteinemia, and hyperglobulinemia, which present themselves as strong clinical markers of visceral leishmaniasis associated with the signs previously reported.

【 授权许可】

Unknown