Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical

Laboratório Central do Pará, Belém¹  Centro de Saúde Escola do Marco, Belém¹  Cardoso, Ninarosa Calzavara¹  Sousa, Maísa Silva¹  Costa, Maurimélia Mesquita¹  Lopes, Maria Luíza¹  Loureiro, Edvaldo Carlos Brito¹  Universidade Federal do Pará, Belém¹  Instituto Evandro Chagas, Belém¹  Lima, George Leandro Ferreira¹  Lima, Karla Valéria Batista¹ 

关键词: Tuberculosis;    Nested-PCR;    Mycobacterium tuberculosis;    Molecular diagnosis;    Antigen b;   

DOI  :  10.1590/S0037-86822007000200013

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

The polymerase chain reaction used for amplifying an internal sequence of a previously amplified fragment (nested-PCR) was investigated as a complementary alternative for searching for alcohol-acid resistant bacilli and Mycobacterium tuberculosis cultures in Lowenstein-Jensen medium. 144 sputum samples were investigated from patients with suspected tuberculosis that were sent to the Tuberculosis Laboratory of the Evandro Chagas Institute in Belém, between June 2002 and December 2003. From the 144 samples, 121 were characterized as tuberculosis: 119 were positive in cultures, 95 under bacilloscopy and 128 using nested-PCR. The sensibility of the nested-PCR was 96% (116/121), while the specificity was 48% (11/23). Nested-PCR may be a complementary tool for diagnosing tuberculosis, since it presents sensitivity equivalent to that of cultures. However, further evaluations are needed with the aim of minimizing the number of false-positive results.

【 授权许可】

Unknown