Immunodiagnosis of human neurocysticercosis by using semi-purified scolex antigens from Taenia solium cysticerci

Revista da Sociedade Brasileira de Medicina Tropical

Nascimento, Evaldo¹  Universidade Federal de Minas Gerais, Belo Horizonte¹  Iudici Neto, Francesco¹  Araújo, Ricardo Nascimento¹  Fundação Ezequiel Dias, Belo Horizonte¹  Pianetti-Filho, Geraldo¹ 

关键词: Taenia solium;    Human neurocysticercosis;    Semi-purified proteins;    Immunodiagnosis;   

DOI  :  10.1590/S0037-86822007000200004

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Crude antigen and semi-purified proteins from scolices of Taenia solium cysticerci were evaluated for the immunodiagnosis of human neurocysticercosis neurocysticercosis. Semi-purified proteins obtained by electrophoresis on polyacrylamide gel and by electroelution were tested by means of the immunoenzymatic reaction against sera from normal individuals and from patients with neurocysticercosis or other parasitic diseases. The 100kDa protein provided 100% sensitivity and specificity in the immunodiagnosis. When 95 or 26kDa proteins were used, 95 and 100% sensitivity and specificity were obtained, respectively. The assays involving crude antigen and sera from normal individuals or from patients with neurocysticercosis, diluted to 1:256, gave excellent agreement with those in which 100, 95 or 26kDa proteins were tested against the same serum samples diluted to 1:64. (Kappa: 0.95 to 1.00). Crude scolex antigen may be useful for serological screening, while 100, 95 or 26kDa protein can be used in confirmatory tests on neurocysticercosis-positive cases.

【 授权许可】

Unknown