In vitro assessmentof Tribulus terrestrisaqueous extract and Benzoxacin fractionagainst Helicobacter pylori isolates from biopsy samples of Iranianpatients

Novelty in Biomedicine

Sahar Jahangiti³  Mohammad Kamali Nejad¹  Hossain Goudarzi²  Mehrdad Gholami³  Siavash Naseri Moghadam⁴  Mojdeh Hakemi vala⁵ 

[1] Pharmaceutics department, Pharmacology school, Shahid Beheshti university of medical sciences (SBMU) Tehran/Iran;Microbiology department, Medical school, Shahid Beheshti university of Medical Sciences Tehran/ Iran;Microbiology department, Medical school, Shahid Beheshti university of medical sciences(SBMU) Tehran/Iran;Digestive Diseases Research Center, Shariati Hospital , Tehran University of Medical Sciences Tehran/Iran;Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences

关键词: Helicobacter pylori;    Tribulus terrestris;    Benzoxacin;    antibacterial resistance;   

DOI  :  

学科分类：生物科学（综合）

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Background & Objectives:  Helicobacter pylori(Hp) is related to gastritis, gastric ulcer, duodenal ulcer, and mucosal carcinoma. Emergence of multidrug resistant Hp strains encouraged the researchers to find new effective drugs. Especially medicinal herbs and plants which usually shows less side effects. The aim of this study was in vitro assessment of anti Hp activity of total extract ofTribulus terrestris (T. terrestrisBenzoxacin, a local Iranian medicinal plant and its fractionBenzoxacin.Methods : total aqueous extract of aerial parts of the plant was prepared and liquid extraction with petroleum ether was used to separate its components. LC/MS system proved the existence of Benzoxazine derivative in the water fraction and the third's fraction. Anti (Hp) effects of total extract and its third fraction were examined by cup plate method and using standard MacFarland. 50 biopsy samples of antrum were detected from patients who were endoscopic candidates in Milad and Fayazbakhsh  hospitals of Tehran during 2011. All samples were isolated, diagnosed based on standard methods and biochemical tests and confirmed by PCR method for ureC gene, too.  Different dilutions (250, 500,750 and 1000 mg/ml) of total extract were prepared. Clarythromycin (Cl r ) E-test strips and an identified Hp OC1096 was used, simultaneously. Results:Of 50 biopsy samples, 12 Hp strains were isolated. Rapid urease test were positive in all expect one biopsy sample. Existence of ureC gene in all isolates were confirmed expect one strain by PCR. By cup plate method, resistant to concentrations of 1000 and 750mg/ml were detected in 50% of Hp isolates and 66.6% of them were resistant to concentrations 250 and 500 mg/ml .Also, 83.3% of Hp strains were resistant to Benzoxacin fraction. Clarythromycin sensitivity detected in 83% of Hp isolates, simultaneously. Conclusion:This study was done as a pilot study for in vitro evaluation of antibacterial effect of total extract ofT. terrestrisby cup plate method. Existence of high resistant rate (≥50%) to different concentrationsT. terrestrisaqueous extract seems doing test on more Hp strains  in future studies is highly  recommended .  In contrast of the similarity of Benzoxazin structure to Ofloxacin, existence of 83.3% of resistance among tested isolates showed no anti Hp effectiveness of this fraction.  

【 授权许可】

Unknown