In vitro cytotoxicity assessment of an orthodontic composite containing titanium-dioxide nano-particles

Journal of Dental Research, Dental Clinics, Dental Prospects

Mohammad Ramezani⁴  Farzaneh Ahrari⁵  Farzin Heravi³  Maryam Poosti²  Mohsen Hosseini⁶  Arezoo Shajiei¹ 

[1] Assistant professor, Department of Orthodontics, School of Dentistry, Islamic Azad University, Tehran, Iran;Dental Research Center, School of D;Pharmaceutical and Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran;Achool of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

关键词: Adhesive;    biocompatibility;    cytotoxicity;    nano-particles;    orthodontics;    titanium dioxide;   

DOI  :  

学科分类：农业科学（综合）

来源: Tabriz University of Medical Sciences * Faculty of Dentistry

【 摘 要 】

Background and aims. Incorporation of nano-particles to orthodontic bonding systems has been considered to prevent enamel demineralization around appliances. This study investigated cytotoxicity of Transbond XT adhesive containing 1 wt% titanium dioxide (TiO2) nano-particles.Materials and methods. Ten composite disks were prepared from each of the conventional and TiO2-containg composites and aged for 1, 3, 5, 7 and 14 days in Dulbecco’s Modified Eagle’s Medium (DMEM). The extracts were obtained and exposed to culture media of human gingival fibroblasts (HGF) and mouse L929 fibroblasts. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results. Both adhesives were moderately toxic for HGF cells on the first day of the experiment, but the TiO2-containing adhesive produced significantly lower toxicity than the pure adhesive (P 0.05). There was a significant reduction in cell toxicity with increasing pre-incubation time (P < 0.001). L929 cells showed similar toxicity trends, but lower sensitivity to detect cytotoxicity of dental composites.Conclusion. The orthodontic adhesive containing TiO2 nano-particles indicated comparable or even lower toxicity than its nano-particle-free counterpart, indicating that incorporation of 1 wt% TiO2 nano-particles to the composite structure does not result in additional health hazards compared to that occurring with the pure adhesive.

【 授权许可】

Unknown