Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells

Revista Brasileira de Farmacognosia

Universidade Federal de São Paulo, Santos, Brazil¹  Valverde, Alessandra L.¹  Universidade Federal do Piauí, Parnaíba, Brazil¹  Pinheiro, Leandro S.¹  Marinho-Filho, José Delano B.¹  Universidade de São Paulo, São Paulo, Brazil¹  Epifanio, Rosângela A.¹  Amorim, Juliana W.¹  Ferreira, Elthon G.¹  Wilke, Diego V.¹  Abreu, Paula A.¹  Universidade Federal do Ceará, Fortaleza, Brazil¹  Strasbourg University, Strasbourg, France¹  Araujo, Ana J.¹  Costa-Lotufo, Letícia V.¹  Jimenez, Paula C.¹  Universidade Federal Fluminense, Niterói, Brazil¹  Ribeiro, Carlos Margo R.¹ 

关键词:  Gorgonian coral;    Marine natural products;    Cytotoxicity;    Quinone;    ROS;   

DOI  :  10.1016/j.bjp.2015.07.020

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress.

【 授权许可】

Unknown