Evaluation of antinociceptive and anti-inflammatory activities of extract and fractions of Eugenia jambolana root bark and isolation of phytoconstituents

Revista Brasileira de Farmacognosia

Jadavpur University, India¹  Nitte University, India¹  Mandal, Shubhash C.¹  Saha, Santanu¹  Kodangala, Chandrashekar¹  Shastry, Shashidhara C.¹  Manipal University, India¹  Subrahmanyam, E. V. S¹  Srinivas College of Pharmacy, Vallachil, India¹ 

关键词: adjuvant induced arthritis;    anti-inflammatory;    antinociceptive;    formalin induced nociception;    Eugenia jambolana;    Myrtaceae;   

DOI  :  

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Eugenia jambolana Lam., Myrtaceae, is a widely distributed and traditionally well known plant in India. The root bark of the plant was extracted with ethanol and then successively fractionated into petroleum ether fraction, chloroform fraction, n-butanol fraction and methanol fraction. The extract and fractions of the plant material were evaluated for the antinociceptive activity by acetic acid-induced writhing test and formalin-induced nociception test, and anti-inflammatory activity was screened by carrageenan-induced rat paw edema, cotton pellet induced granuloma formation and adjuvant induced arthritis in rat models. The test materials showed the antinociceptive and anti-inflammatory effect in dose dependent manner and the petroleum ether fraction was found to be most potent among the test materials. At 400 mg/kg b.w., p.o. dose petroleum ether fraction significantly inhibited 54.28% writhing response and 73.77% formalin induced nociception in mice. The fraction with same dose showed significant 79.31% inhibition of carrageenan-induced rat paw edema, 57.78% anti-proliferative effect and 77.93% inhibition of adjuvant induced arthritis. The bioactive petroleum ether fraction was then subjected to column chromatography which led to isolate three compounds, namely, β-sitosterol, stigmasterol and lupeol. These compounds were characterized and identified by IR, 1H NMR, 13C NMR and mass spectroscopy.

【 授权许可】

Unknown