Combretum lanceolatum flowers extract shows antidiabetic activity through activation of AMPK by quercetin

Revista Brasileira de Farmacognosia

Kawashita, Nair Honda¹  Souza, Damiana Luiza Pereira de¹  Siqueira, Juliany Torres¹  Silva, Virginia Claudia da¹  Universidade Federal de Mato Grosso, Brazil¹  Andrade, Claudia Marlise Balbinotti¹  Dechandt, Carlos Roberto Porto¹  Baviera, Amanda Martins¹  Araujo, Lais Cobianchi Junqueira¹  Sousa Junior, Paulo Teixeira de¹ 

关键词: AMPK;    antidiabetic activity;    Combretum lanceolatum;    metformin;    quercetin;    streptozotocin-diabetic rats;   

DOI  :  

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

The present study evaluated the antidiabetic activity of the Combretum lanceolatum Pohl ex Eichler, Combretaceae, flowers extract (ClEtOH) in diabetic rats. Streptozotocin-diabetic rats were divided into four groups: diabetic control, diabetic treated with 500 mg/kg of metformin and diabetic treated with 250 or 500 mg/kg of ClEtOH for 21 days. The treatment of diabetic rats with 500 mg/kg of ClEtOH promoted an increase in the weight of liver, white adipose tissues and skeletal muscles, improving body weight gain. Diabetic rats treated with 500 mg/kg of ClEtOH also presented reduction in glycemia, glycosuria and urinary urea levels, and increase in liver glycogen content. HPLC chromatogram showed that quercetin is the major compound in the extract. The phosphorylation levels of adenosine monophosphate-activated protein kinase were increased in liver slices incubated in vitro with 50 µg/mL of ClEtOH, similarly to the incubation with metformin (50 µg/mL) or quercetin (10 µg/mL). The antihyperglycemic effect of ClEtOH was similar to that of metformin and appears to be through inhibition of gluconeogenesis, since urinary urea was reduced and skeletal muscle mass was increased. These data indicate that the antidiabetic activity of the Combretum lanceolatum extract could be mediated, at least in part, through activation of adenosine monophosphateactivated protein kinase by quercetin.

【 授权许可】

Unknown