Antimycobacterial activity evaluation and MIC determination of liophilizated hydroalcoholic extracts of Bixa orellana L., Bixaceae

Revista Brasileira de Farmacognosia

Chavasco, Jorge K.¹  Silva, Renata B.¹  Universidade Federal de Alfenas, Alfenas, Brasil¹  Universidade José do Rosário Vellano, Alfenas, Brasil¹  Almeida, Cristina R.¹  Chavasco, Juliana M.¹ 

关键词: Mycobacterium tuberculosis;    Bixa orellana;    Bixaceae.;   

DOI  :  10.1590/S0102-695X2010000200006

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Bixa orellana is used in popular medicine against several diseases. Extracts of its fruit, root and leaf presented antimicrobial activity, while seed extract showed negative results. This study aimed at verifying the antimycobacterial activity of B. orellanalyophilized hydroalcoholic extracts over Mycobacterium tuberculosis, and determine the Minimum Inhibitory Concentration against five bacteria. Antimycobacterial activity was determined by diffusion technique, while MIC was assessed by diffusion and colorimetric analysis. MICs were 0.3, 0.5 and 0.2 mg/mL respectively, for leaf, root and stem extracts, against M. tuberculosis. Stem's extract showed 1.2 mg/mL for B. cereus, 1.53 mg/mL for S. aureusand S. typhimurium, 4.50 mg/mL for P. aeruginosaand 8.01 mg/mL for P. mirabillis.Leaf extracts showed 0.66 mg/mL for P. aeruginosa, 0.94 mg/mL for P. mirabillis, 1.88 mg/mL for S. aureus, 3.95 mg/mL for B. cereusand 8.37 mg/mL for S. typhimurium. Root's extracts showed 0.25 mg/mL for P. aeruginosa,0.31 mg/mL for S. aureus, 0.62 mg/mL for S. typhimuriumand 3.00 mg/mL for B. cereusand P. mirabillis. Leaf and stem extracts showed antimycobacterial activity. MICs were lower in colorimetric analysis than in agar diffusion. Extracts revealed bacteriostatic activity against the five bacterial.

【 授权许可】

Unknown